Olyset Plus

The success and popularity of Long Lasting Insecticidal net (LLIN) campaigns combined with the agricultural use of insecticides has contributed to the emergence of pyrethroid insecticide resistance among mosquito populations in Africa and other malaria endemic countries. Olyset Plus is the ‘bite-back’ solution with enhanced knockdown and kill against pyrethroid-resistant mosquitoes.

Olyset Plus is a durable polyethylene net containing 2% permethrin combined with 1% of the synergist PBO (piperonyl butoxide), which is incorporated into all the fibres on each side including the roof. Using patented slow-release technology, the net fibres release the two ingredients at a constant ratio of 2:1, ensuring optimum efficacy against mosquitoes, including those that are resistant, in even the toughest conditions.

For more information on insecticide resistance please visit Insecticide Resistance Action Committee’s (IRAC) guidance on ‘Prevention and Management of Insecticide Resistance In Vectors of Public Health Importance’

How does Olyset Plus work?

Olyset Plus Regeneration

Building on Olyset Net technology, liquid permethrin is released into tough polyethylene fibres, but with the addition of PBO to boost efficacy. The rate in which PBO and permethrin migrate from the fibre’s internal reservoir to the surface of the net has been adjusted in Olyset Plus to provide rapid regeneration, making the net fully active again within 1-2 days after washing.

We believe that in areas where resistant mosquitoes have been found, rapid regeneration after washing of both synergist and insecticide is required to ensure that LLINs repel, kill and prevent mosquitos from biting adults and young children.

What is Piperonyl Butoxide?

Piperonyl butoxide (PBO) is a synergist which has been safely used for over 50 years to enhance the performance of pyrethroid insecticides, especially in household aerosols. When combined with permethrin, the protective effect of the insecticide is dramatically increased.

How does Piperonyl Butoxide combat resistance?

All insects, whether susceptible or resistant, have enzymes that can metabolise insecticides into harmless components. Some of these enzymes, known as cytochrome P450s, are powerful oxidising agents that can detoxify an active ingredient before the desired effect is achieved. By adding PBO, this action can be blocked. The synergist inhibits the breakdown or the metabolism of insecticides in resistant insects, rendering the insecticide more effective; while at the same time the activity of pyrethroids to susceptible insects is increased. For this reason, adding PBO to an LLIN is beneficial even in areas where there is no insecticide resistance.

Geography

Distribution of pyrethroid resistance in Africa using data collected between 2000 to 2010

Due to the excellent results from LLIN campaigns the adoption of pyrethroid treated nets as a key intervention in the battle against malaria has reached new heights. In many endemic countries, usage had reached 80% by 2011. Yet the pressure of using a single class of insecticides, pyrethroids, in all LNs as well as for Indoor Residual Spraying and agriculture has resulted in insecticide resistance in many parts of the world, particularly in Africa.

Distribution of pyrethroid resistance in Africa using data collected between 2000 to 2010

Reference: Ranson et al, Trends in Parasitology, Feb 2011, Vol 27, No.2

Olyset Plus is the first and only net on the market to incorporate PBO into every fibre and all surfaces of the net, providing enhanced knockdown and kill against pyrethroid-resistant mosquitoes.

Field Data/Biological

Rapid regeneration time

WHO cone tests to evaluate speed of regeneration of Olyset Plus against field-caught, pyrethroid-resistant mosquitoes. Tests were performed one day after washing.

Ref : Sumitomo Chemical Health and Crop Sciences Laboratory Report, 2011

Rapid regeneration time

2 days after 20 washes against pyrethroid resistant mosquitoes

Ref : Sumitomo Chemical Health and Crop Sciences Laboratory Report, 2011

2 days after 20 washes against pyrethroid resistant mosquitoes

Olyset Plus vs. a Polyester LLIN against field caught resistant mosquitoes

Ref : Sumitomo Chemical Health and Crop Sciences Laboratory Report, 2011

Olyset Plus vs. a Polyester LLIN against field caught resistant mosquitoes

Blood Feeding Inhibition in hut trials against resistant mosquitoes in Benin

Ref : C.Pennetier,V.Corbel et al, 2010

Blood Feeding Inhibition in hut trials against resistant mosquitoes in Benin

Experimental hut trials in Cameroon: Olyset Net and Olyset Plus

Ref : C.Pennetier, V.Corbel et al. 2010

Experimental hut trials in Cameroon: Olyset Net and Olyset Plus

WHOPES Phase II experimental hut trials, Malanville, Benin

Ref : CREC/IRD WHOPES Phase II report 31/1/2012

WHOPES Phase II experimental hut trials, Malanville, Benin

Research & Reports

Pennetier C, Bouraima A, Chnadre F, Piameu M, Etang J, Rossignol M, Sidick I, Zogo B, Lacroix MN, Yadav R, Pigeon O, Corbel V

Due to the rapid extension of pyrethroid resistance in malaria vectors worldwide, manufacturers are developing new vector control tools including insecticide mixtures containing at least two active ingredients with different modes of action as part of resistance management. Olyset Plus is a new long lasting insecticidal net (LLIN) incorporating permethrin and a synergist, piperonyl butoxide (PBO) into its fibres in order to counteract metabolic based pyrethroid resistance of mosquitoes. In this study we evaluated the efficacy of Olyset Plus both in laboratory and field against susceptble and multi resistanct malaria vectors and compared with Olyset Net which is a permethrin incorporated into polyethylene net.

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Filiz Gunay, Mehmet Karakus, Gizem Oguz, Mert Dogan, Yasemen Karakaya, Gokhan Ergan, Sinan Kaynas, Ozge Erisoz Kasap, Yusuf Ozbel and Bulent Alten

The aim of this study was to measure the protective efficacy of Olyset Plus, a new long-lasting factory-treated insecticidal net (LLIN) incorporated with 2% permethrin and 1% of the synergist piperonyl butoxide (PBO), against cutaneous leishmaniasis (CL) transmission under field conditions. A village-scale trial, promoting the use of LLIN by the local inhabitants of the study area, was conducted as a pilot study in a new hyperendemic focus of CL caused by a Leishmania infantum/L. donovani hybrid parasite transmitted by proven vector species Phlebotomus tobbi in Cukurova Plain, Adana, Turkey, between May, 2013 and May, 2014

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Thomas S Churcher, Natalie Lissenden, Jamie T Griffin, Eve Worrall, Hilary Ranson

Churcher et al. also show that the combination bednets may provide some additional protection against resistant mosquitoes and reduce the number of malaria infections in some cases. The experiments show a simple test could help health officials determine which type of net would be most beneficial. The experiments and the model Churcher et al. created also may help scientists studying how to prevent increased spread of malaria in communities where mosquitoes are becoming resistant to insecticide-treated nets.

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K. Gunasekaran, S. S. Sahu, T. Vijayakumar, K. Vaidyanathan, P. Jambulingam

Olyset Plus is a new generation long-lasting insecticidal net that incorporates two active ingredients, the pyrethroid insecticide permethrin and the oxidase synergist piperonyl butoxide. Mixture nets such as this may have application against resistant mosquitoes, particularly those whose resistance is based on oxidative metabolism. There may also be enhanced activity against susceptible mosquitoes since mixed function oxidases are involved in many metabolic activities including activation of many substances to form their bio-active compounds, and hence evaluation was done against a conventional permethrin treated ITN as well as Olyset Net LN. Olyset Plus LN was made available by the WHOPES for Phase II evaluation.

The efficacy of Olyset Plus LN was evaluated in experimental huts against a wild, free flying susceptible population of Anopheles fluviatilis sensu lato in terms of mortality, deterrence, blood-feeding inhibition and induced exophily in Odisha state, East-central India following the WHO guidelines.

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Technical Brochure 2018 English Download PDF
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Folheto Técnico 2013 Português Download PDF
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